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Such codon usage will depend on the cell type selected.
39. The chimeric antigen receptor of claim 38, wherein the intracellular signaling domain further comprises an intracellular signaling region of
a T cell costimulatory molecule. In some embodiments, the anti-GPC3 Adnectin comprises the amino acid sequence set forth in SEQ ID NO: 3, wherein the BC, DE, and FG loops comprise the amino acid sequences set forth in SEQ ID NOs: 99, 100 and 210, respectively, wherein the BC loop has 0, 1,
2, 3, 4, 5, or 6 amino acid substitutions, such as conservative amino acid substitutions, and the DE
loop has 0, 1, 2 or 3 amino acid substitutions, such as a
conservative amino acid substitution. ”